DNASE1 anticorps (HRP)

N° du produit ABIN7565874

Cet anticorps anti-DNASE1 est un anticorps Lapin Polyclonal détectant DNASE1 dans WB, ELISA et IP. Adapté pour Boeuf (Vache).

Aperçu rapide pour DNASE1 anticorps (HRP) (ABIN7565874)

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNASE1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-DNASE1 anticorps (HRP)

Fonction: Deoxyribonuclease I Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].

Attributs du produit: Synonyms: rabbit anti-Deoxyribonuclease I Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

Purification: Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Deoxyribonuclease I [Bovine Pancreas]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Deoxyribonuclease I Peroxidase Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Deoxyribonuclease I [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur DNASE1

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Autre désignation: DNASE1, DNL1

Sujet: Background: Deoxyribonuclease I (DNase I) is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.

ID gène: 282217

NCBI Accession: NP_776959

UniProt: P00639